ACORP Appendix 4  
Antemortem Specimen Collection
Version 3

1.	Blood Collection.  Will blood be collected from live animals (anesthetized or awake) as a part of this proposal?  
	No.  Proceed to item 3.  
	Yes, but all collections are described in Appendix 2, “Antibody Production”, so no further information need be provided here; proceed to item 3.  
	Yes.  Complete the table below; then proceed to item 2.  

Site and Method of blood collection	Amount of blood collected, expressed as volume (ml) and % of body weight (assume 1 ml of blood weighs 1 gram)	Number of blood collections	Interval between collections	

				

2.	Use of Anesthetics, Tranquilizers, or Analgesics for Blood Collection.  Will anesthetics, tranquilizers, or analgesics be used to prevent pain or stress during collection of blood described in item 1 above?  
	No.  Justify the omission of pain-relieving agents (either scientifically or because the collection method involves no or momentary pain) and completely describe the physical restraint that will be used during collection here: 

	Yes.  Complete the following table, then proceed to item 3.  

Anesthetic, tranquilizer, or analgesic agent	Dose (mg/kg) & volume (ml)	Route	Frequency	

				

3.	Other Tissue Collection.  Will other body fluids (e.g. cerebrospinal fluid, peritoneal fluid, urine) or tissues be collected from live animals (awake or anesthetized) as a part of this protocol?  
	No.  Proceed to item 5.  
	Yes.  Complete the following table; then proceed to item 4.  

Tissue or fluid collected	Site & method of collection	Amount (g) or volume (ml)	Number of collections	Interval between collections	

					

4.	Use of Anesthetics, Tranquilizers, or Analgesics for Collection of Fluids or Tissues.  Will anesthetics, tranquilizers, or analgesics be used to prevent pain or stress during collection of body fluids or tissues described in item 3 above?  
	No.  Justify the omission of pain-relieving agents (either scientifically or because the collection method involves no or momentary pain) and completely describe the physical restraint that will be used during collection here, then go to item 5:
  
	Yes.  Complete the following table, then go to item 5.  

Anesthetic, tranquilizer, or analgesic agent	Dose (mg/kg) & volume (ml)	Route	Frequency	

				

5.	Proceed to item S on the ACORP.